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Volume 2 Issue 2, 2021
Alternative Splicing of AT1G29800 and AT4G06634 Genes and its Effect on Protein Structure and Functionality in Arabidopsis thaliana
Umme Qulsum, Md. Thoufic Anam Azad, Toshifumi Tsukahara
Abstract: Alternative splicing is a post and co-transcriptional regulation of gene expression. Recently it has been revealed that not only PPR (Pentatricopeptide repeat) family genes but also other additional family genes MORF/RIP, ORRM and Zinc finger are involved in RNA editing. The aim of this study is to find out the tissue-specific expression and alternative splicing of Zinc finger family genes AT1G29800 and AT4G06634 and their effect on protein structure and functionality. AT1G29800 and AT4G06634 genes are functionally very importance which are probably alternatively spliced and are located in the protein coding region which is tentatively determined using the Arabidopsis database. Tissue-specific alternative splicing in AT1G29800 with intron retention and AT4G06634 with 5' alternative splice site genes of zinc finger family was confirmed by PCR and sanger sequencing. This study suggests that tissue-specific expression of different alternatively spliced transcript happen even in different developmental stages.
Present Status and Potentiality of Biofloc Technology in Bangladesh
Kamruzzaman, Mst. Shahinur Khatun, Taiba Akter Laboni, Md. Mizanur Rahman, Md Amirul Islam, Obaidur Rahman, Most. Shakila Sarmin, Md. Harun, Md. Mahabubur Rahman, Md. Akhtarul Islam and Md. Yeamin Hossain
Abstract: Biofloc technology stands as the blue revolution in aquaculture, presenting a new technique for improving water quality and utilizing feed wastes in the aquaculture system. The research aimed to assess the current status and potentiality of Biofloc fish farming in Bangladesh. Data were collected during July 2021 to December 2022 across various districts in Bangladesh. Data from a total of ten farmers were included used in the study who were involved in Biofloc fish farming technology (BFT). The majority of the farmers were within the age category of 30 to 40 years. From the survey, 100% educated individuals were interested in BFT. Additionally, majority of farmers received training directly or indirectly via YouTube. Farmers faced challenges such as diseases i.e., fungal disease, fin and tail rot disease, and dropsy. the survey also revealed the other problems like lack of proper knowledge about technology, inappropriate species selection, and elevated operational costs. This small-scale fish farming was not be a profitable venture. However, the proper designing and management of the Biofloc unit should increase the fish productivity and also established a platform for knowledge sharing and experience exchange among farmers who have successfully implemented Biofloc systems to minimize the loss in this sector.
Hematological Profile of Indigenous Sheep in Rajshahi Metropolitan Area of Bangladesh
Most. Nahida Khatun, Rehana Parvin, Rubina Khatun, Afsana Akta, Subarna Rani Kundu, Shah M. A. Rauf and Hossain M. Golbar
Abstract: Hematological profiles are important candidates to assess health and disease of animals. We investigated the hematological parameters of indigenous sheep (Ovis aries). Blood samples collected from young (group-1), adult male (group-2) and adult female (group-3) sheep were analyzed for total red blood corpuscle (RBC), hemoglobin (Hb) content, packed cell volume (PCV), total white blood cell (WBC), differential leukocytes and erythrocyte sedimentation rate (ESR). Total RBC count was higher in group-3 (4.81±1.98×106/µL) compared to group-1 (4.73±2.02×106/µL) and group-2 (3.12±1.22×106/µL). Hemoglobin content was significantly higher in group-3 (10.70±0.35 g/dL) than group-1 (9.25±0.50 g/dL) (p<0.05) and group-2 (9.98±1.79 g/dL). However, PCV was slightly higher in group-1 (35.25±8.06%) and group-3 (35.00±4.83%) than group-2 (32.75±4.65%). By contrast, total WBC count was higher in group-2 (13.65±6.34×103/µL) than in group-1 (11.53±1.36×103/µL) and group-3 (9.49±4.08×103/µL). Differential leukocyte count showed significant decrease of neutrophils in group-2 (17.50±10.08%) compared to group-1 (30.50±3.32%) and group-3 (33.25±8.14%). Although eosinophil count was significantly different among the groups, the number was significantly higher in group-2 (24.00±11.11%) than in group-1 (8.50±2.65%) and group-3 (12.25±2.22%). Lymphocyte (50.25±4.43%-52.00±6.06%), monocyte (4.00±3.65–8.50±2.65%) and basophil (0.25±0.50-0.50±0.58%) count did not differ among the groups. Finally, ESR values were very low (0.32±0.01-0.67±0.01).
Growth, Condition and Spawning Season of Chanda nama (Hamilton, 1822) in a Wetland Ecosystem (Gajner beel): Recommendation for its Sustainable Management
Md. Motiour Rahman Khan, Taiba Akter Laboni, Mst. Shahinur Khatun, Md. Ashekur Rahman, Md. Akhtarul Islam and Md. Yeamin Hossain
Abstract: This research presents a comprehensive analysis of the population structure, growth patterns, condition factors (including allometric KA, Fulton KF, relative KR, and relative weight WR), spawning and peak spawning season through GSI (gonadosomatic index), along with the size at sexual maturity (Lm) of Chanda nama in the wetland ecosystem of Gajner beel, Bangladesh from May 2019 to October 2021. A total of 507 fish samples were randomly collected monthly by commercial fishers using cast nets, gill nets, and square lift nets. The specimens exhibited a total length (TL) variation ranging from 2.6 to 7.2 cm. The estimated allometric coefficient (b) suggested isometric growth (b = 3.089). Additionally, relative weight WR (101.08± 10.28) did not significantly differ from the expected value of 100 (p<0.0001). Based on TL vs. GSI results and considering Lmax, the calculated Lm was found to be 3.9 cm and 4.41 cm. The spawning period was observed from April to October, with the peak season in June to July. The research recommends the establishment of a fishing ban during June-July, and a complete prohibition of fishing until the individuals attain sexual maturity. These measures aim to conserve C. nama populations in Gajner beel and its adjacent ecosystems.
Status of Fish Availability and Fishing Gears of Nabaganga River at Jhenaidah District, Bangladesh
M. R. Nasim and S. N. Jahan
Abstract: The study was conducted from December 2022 to November 2023 to investigate the fish availability and fishing gears in the Nabaganga River at Jhenaidah district. Data were collected through questionnaire, interview and direct observation method. Species of the studied river, gear type and CPUE were estimated. During the study period, 27 species of fish under 7 orders and 14 families were identified in the Nabaganga River. Cypriniformes was the most dominant order constituting 40.74% of the total fish population followed by the Perciformes (22.22%), Siluriformes (14.81%), Clupeiformes (7.41%), Channiformes (7.41%), Beloniformes (3.7%) and Mastacembeliformes (3.7%). Population trends among the 27 species, 25.93% were decreasing, 33.33% were declining and 40.74% were unknown. The study disclosed 6 types of fishing gears which are broadly categorized as nets, traps, and hooks and lines. The average catch composition was found for Ber jal (12.5±0.90 kg/day) followed by Current jal (8.0±1.80 kg/day). The catch composition of Jhaki jal, Thela jal, Polo and Chip borshi were 4.25±0.5 kg/day, 2.75±1.32 kg/day, 1.25±0.05 kg/day and 1.95±0.04 kg/day, respectively. The lowest catch composition was measured for Polo (1.25±0.05kg/day). Negative activities, like destructive gears, agrochemical uses, and barrage construction were identified as causes for declining fish availability in the Nabaganga River.